Ka hoʻohana ʻana i ka ultrafiltration i ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe protein

Application of ultrafiltration in protein separation and purification1

ʻO ka ʻenehana Ultrafiltration kahi ʻenehana hoʻokaʻawale kiʻekiʻe a kiʻekiʻe.Loaʻa iā ia nā hiʻohiʻona o ke kaʻina hana maʻalahi, ka pōmaikaʻi waiwai kiʻekiʻe, ʻaʻohe hoʻololi o ka pae, ka nui o ka hoʻokaʻawale ʻana, ka mālama ʻana i ka ikehu, ka hana kiʻekiʻe, ʻaʻohe pollution lua, ka hana mau i ka lumi wela a pēlā aku.I kēia lā, ua nīnau ʻo luna ʻo Yang mai Beijing e pili ana i kā mākou mea hana ultrafiltration no ka hoʻomaʻemaʻe protein a kamaʻilio kikoʻī me kā mākou ʻenehana.I kēia manawa, e hoʻopuka ka mea hoʻoponopono o ka hui Shandong Bona i ka noi o ka ultrafiltration i ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe protein.

1. No ka protein desalination, dealcoholization a me ka noonoo
ʻO nā noi nui loa o ka ultrafiltration i ka hoʻomaʻemaʻe ʻana i nā protein he desalting a me ka noʻonoʻo.Hōʻike ʻia ke ʻano o ka Ultrafiltration i ka desalination a me ka noʻonoʻo ʻana e ka nui o ka batch volume, ka manawa hana pōkole a me ka hana kiʻekiʻe o ka hoʻihoʻi ʻana i ka protein.ʻO ke ʻano kuʻuna o ka steric exclusion chromatography e wehe i nā mea like ʻole mai nā protein ua hoʻololi ʻia e ka ʻenehana ultrafiltration hou, a ua lilo ia i ʻenehana nui no ka desalination protein, dealcoholization a me ka neʻe ʻana i kēia lā.I kēia mau makahiki i hala iho nei, ua hoʻohana nui ʻia ka ʻenehana ultrafiltration i ka desalination a me ka hoʻihoʻi ʻana i nā protein waiwai waiwai nui i ka cheese whey a me ka soybean whey.ʻO ka lactose a me nā paʻakai a me nā mea ʻē aʻe i loko o ka protein, a me nā pono maoli o ka hoʻopau maikaʻi ʻana i ka desalting, de-alcoholization a me ka neʻe ʻana o nā protein.Hiki i ka hoʻohana ʻana i ka ʻenehana ultrafiltration ke hoʻopili i nā serospecies immunoglobulins e hoʻokō i ka koi maoli o ka hua protein.

2. No ka haʻihaʻi protein
ʻO ka haʻihaʻi protein e pili ana i ke kaʻina hana o ka hoʻokaʻawale ʻana i kēlā me kēia ʻāpana ʻāpana protein ma ka ʻāpana e like me ka ʻokoʻa o nā waiwai kino a me nā waiwai kemika (e like me ke kaumaha molekala pili, ke kiko isoelectric, hydrophobicity, etc.) o kēlā me kēia ʻāpana protein i loko o ka wai hānai.ʻO ka chromatography gel kekahi o nā ʻano hana maʻamau no ka hoʻokaʻawale ʻana i nā macromolecules biological (ʻoi aku ka nui o nā protein).Ke hoʻohālikelike ʻia me ka chromatography kuʻuna, loaʻa i ka ʻenehana hoʻokaʻawale ultrafiltration kahi manaʻo maikaʻi o ka noi i ka fractionation a me ka hana ʻenehana o nā protein a me nā enzymes me ka waiwai waiwai nui ma muli o kāna kumu kūʻai haʻahaʻa a maʻalahi hoʻi ka hoʻonui.ʻO ka hua keʻokeʻo ka mea makaʻu loa e loaʻa ai ka lysozyme a me ka ovalbumin.I kēia mau lā, hoʻohana pinepine ʻia ka ultrafiltration e hoʻokaʻawale i ka ovalbumin a me ka lysozyme mai ka hua keʻokeʻo.

3. Ka wehe 'ana o Endotoxin
ʻO ka wehe ʻana o Endotoxin kekahi o nā palapala noi nui o ka ʻenehana ultrafiltration i ka hoʻomaʻemaʻe protein.He paʻakikī loa ka hana ʻana o ka endotoxin.I ke kaʻina hana o ka hoʻohana pono, no ka mea, ua maʻalahi ka hui ʻana o ka protein lāʻau lapaʻau e ka ʻōnaehana hōʻike prokaryotic me ka endotoxin i hoʻopuka ʻia e ka paia o ka paia o ka maʻi bacterial, a ʻo ka endotoxin, i ʻike ʻia he pyrogen, he ʻano lipopolysaccharide.Ma hope o ke komo ʻana i ke kino o ke kanaka, hiki iā ia ke hoʻoulu i ke kuni, microcirculation disturbance, endotoxic shock a me nā hōʻailona ʻē aʻe.I mea e pale ai i ke olakino kanaka, pono e hoʻohana i ka ʻenehana ultrafiltration me ka holoʻokoʻa e wehe i nā endotoxins.

ʻOiai ua hoʻohana nui ʻia ka ʻenehana ultrafiltration i ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i nā protein, aia kekahi mau palena.Inā ʻoi aku ka liʻiliʻi o ke kaumaha molekala o nā huahana ʻelua ma mua o 5 mau manawa, ʻaʻole hiki ke hoʻokaʻawale ʻia e ka ultrafiltration.Inā emi ka paona molekala o ka huahana ma mua o 3kD, ʻaʻole hiki ke hoʻopaʻa ʻia e ka ultrafiltration, no ka mea, hana maʻamau ka ultrafiltration ma ka liʻiliʻi loa o ke kaumaha o ka membrane ma 1000 NWML.

Me ka hoʻomau mau ʻana o ka ʻoihana bioengineering, ua kau ʻia nā koi kiʻekiʻe no ka hoʻokaʻawale ʻana i lalo a me ka ʻenehana hoʻomaʻemaʻe.ʻAʻole hiki ke loaʻa nā ʻano kuʻuna o ka vacuum concentration, solvent extraction, dialysis, centrifugation, precipitation and pyrogen removal no ka hoʻokō ʻana i nā pono o ka hana.Hoʻohana nui ʻia ka ʻenehana Ultrafiltration no ka mea ʻoi aku ka maikaʻi o ka hoʻokaʻawale ʻana i ka protein.


Ka manawa hoʻouna: Apr-20-2022
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